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American Society of Microbiology ABMM Sample Questions:

1. A foodborne outbreak is suspected after several individuals who attended a potluck dinner develop nausea, vomiting, and abdominal cramps within 1-6 hours of eating. No fever or diarrhea is reported. The MOST likely bacterial toxin involved is:

A) Clostridium perfringens enterotoxin
B) Shiga toxin
C) Botulinum toxin
D) Staphylococcal enterotoxin

2. A patient with a history of recurrent fungal skin infections presents with a scaly, erythematous rash on their feet. Microscopic examination of skin scrapings treated with potassium hydroxide (KOH) reveals septate hyphae and arthroconidia. The MOST likely genus of the dermatophyte is:

A) Malassezia
B) Candida
C) Aspergillus
D) Trichophyton

3. A research laboratory is developing a novel diagnostic assay for early detection of sepsis. They are investigating the use of host biomarkers that are rapidly upregulated in response to bacterial infection. Which of the following biomarkers has shown promise for differentiating between systemic inflammatory response syndrome (SIRS) and sepsis?

A) Procalcitonin (PCT)
B) Interleukin-6 (IL-6)
C) Erythrocyte sedimentation rate (ESR)
D) C-reactive protein (CRP)

4. According to the current CDC-recommended laboratory algorithm for diagnosing HIV infection in adults and children aged >18 months, what is the recommended initial screening test?

A) HIV RNA nucleic acid test (NAT)
B) HIV-1/HIV-2 antigen/antibody combination immunoassay
C) HIV-1/HIV-2 antibody differentiation immunoassay
D) HIV-1 p24 antigen assay only

5. A patient with a history of intravenous drug use develops a bloodstream infection with Staphylococcus aureus. Antimicrobial susceptibility testing reveals resistance to methicillin. The mechanism of methicillin resistance in Staphylococcus aureus is primarily mediated by:

A) Alteration of penicillin-binding proteins (PBPs).
B) Increased expression of efflux pumps.
C) Production of beta-lactamase enzymes.
D) Acquisition of genes encoding aminoglycoside-modifying enzymes.

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